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The integrity of surface skin cells was evaluated with and without solar simulated irradiation. The integrity of the stratum corneum was significantly lower in individuals treated with P25TiO2NPs under the light in comparison to the ones that received the functionalized nanoparticles. Cell membrane suffering is evident (Fig. 9), and it is in accordance with the ROS levels and macromolecule oxidation found in vitro for the irradiated P25TiO2NPs. Disruption of the superficial skin layer was observed in all animals treated with no functionalized nanoparticles, under irradiation. This data expands the findings by the group of Professors Fubini and Fenoglio, who showed that P25TiO2NPs could impact the lipid structure at the top few microns of the stratum corneum [55]. Control skin under irradiation and without any topic formulation did not show changes in cell structure.

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Titanium dioxide is a versatile and widely used compound that is found in various consumer products due to its unique properties. One of the most common uses of titanium dioxide is as a pigment in paints, coatings, and plastics to provide color, opacity, and UV protection. In addition to its use in consumer products, titanium dioxide is also used in the production of various industrial products, such as catalysts, ceramics, and electronic components.

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